Synovial fluid pretreatment with hyaluronidase facilitates isolation of CD44+ extracellular vesicles
نویسندگان
چکیده
منابع مشابه
Synovial fluid pretreatment with hyaluronidase facilitates isolation of CD44+ extracellular vesicles
Extracellular vesicles (EVs) in synovial fluid (SF) are gaining increased recognition as important factors in joint homeostasis, joint regeneration, and as biomarkers of joint disease. A limited number of studies have investigated EVs in SF samples of patients with joint disease, but knowledge on the role of EVs in healthy joints is lacking. In addition, no standardized protocol is available fo...
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OBJECTIVES To investigate the involvement of the adhesion molecule CD44 in the homing of lymphocytes to synovial tissue, by examining the density of expression and molecular mass of CD44 on rheumatoid synovial fluid lymphocytes. METHODS Twenty patients with rheumatoid arthritis were studied. Peripheral blood and synovial fluid lymphocytes were isolated by Ficoll-Hypaque sedimentation. CD44 ex...
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ژورنال
عنوان ژورنال: Journal of Extracellular Vesicles
سال: 2016
ISSN: 2001-3078
DOI: 10.3402/jev.v5.31751